Photography

Photography

This article describes the process of imaging insect specimens in a museum setting. It will describe general set up techniques for imaging systems and enumerate example systems. This article gives information on how to use related common software as well as tips and trouble shooting. This article will discuss general matters and give system specific examples in related articles.

=Principles of Montage Photography=

=Specimen Preparation= A protocol for preparing ants can be found here.

=Imaging=

Lighting
Problem with image sizes. Delete remaining 4000x Powershot ELPH images of lighting techniques. Text saved in the Ant Wiki Protocol Images folder.

This section will describe general principles of lighting and illustrate various systems. In general LED lights are preferred. Having mixed lighting with different temperatures such as florescent or sunlight will create artifacts in the montaging process and should be avoided.

The goal of good lighting is to reduce the loss of image details caused bright spots and shadows.

We recommend against high intensity microscope lights for digital photography. The fan on the lights creates vibration and the light is difficult to diffuse.

Dome Light
Leica light dome (Leica LED5000 HDI) provides even even diffuse light with very little hassle. It is expensive. Effective homemade systems are listed below.

White Foam
The White Foam technique uses LED reading lights along with the foam found on the bottom of specimen trays. It is assembled using specimen pins making it cheap and easy to assemble. A Styrofoam cup can also be used for this set up.

Diffusion Paper
Diffusion paper is used by photographers to create diffuse light. This system can use the same LED reading lights from the White Foam section. They diffusion paper is used to encircle the specimen.

Window Diffuser
This system uses Window light diffusers cut into strips and wrapped around the specimen. It uses the same LED reading lights reading lights.

Examples of Lighting
The table below lists a series of common lighting problems along with example images, possible causes, and possible solutions.

Specimen Angles

 * A standard set of shots will cover the general anatomy of a specimen. This set of shots is a frontal view of the head, a dorsal shot of the body, a lateral shot of the body, and an image of the specimen labels.


 * Other shots that might be useful are a ventral shot of the body and wing images.


 * Detailed shot of specific body parts can also be taken as required at higher magnifications. For advice on the best angle for detailed anatomy consult an expert in the taxon for the best angle(s) for the image.


 * A large gallery of example ants can be found here.


 * If possible the specimen should be facing left for habitus shots. For specific body parts choose the position that gives the best shot and information.


 * Carefully remove any lint, hair, or debris from the specimen with a pin or small brush. DO NOT do this for types.

Ant Specific Tips
If possible push down legs to unblock the side view. Specifically the later thorax(mesosoma). Do not manipulate legs if imaging a type specimen. Use the eyes to align the head in the same plane. Bring the head to the anterior margin of the clypeus in the the same focal plane. If mandibles are important image them separately. Use the thorax (mesosoma) as your focal plane regardless of head position. If labels are large image them with a hand held camera.
 * Lateral Shots
 * Frontal View of Head
 * Dorsal View
 * Labels

Canon EOS 7D
The Canon EOS 7D allows the user to take images with an interface on the desktop. The interface allows the user to control virtually all of the camera's features. Instructions on how to use the 7D directly from your computer can be found here.

Naming Images
=Image Processing=

Montaging
Montaging/Focus Stacking is the process of combining multiple source images from different focal planes into a single source image.

Software Available
The list of programs below are the programs we are currently aware of. Prices and functionality vary widely. Consider your current and future needs before purchasing.


 * List of Program
 * Auto Montage
 * Zerene Stacker
 * Helicon Focus
 * Combine Z (Freeware)
 * Photoshop CS 6 and higher
 * Leica Application Suite

General Features

 * Source Images - raw images used to create the final image. They should be stored in a single, isolated folder with no other source image sets. Image names should have sequential numbers corresponding to their position in the set of images.


 * Radius/Patch Size - Area of the image that the algorithm processes at any given time. The area increase with the number.


 * Smoothing - This process reduces artifacts left over from the montaging process. Larger number means it will soften the sharpness of images.


 * Retouching - This method allows the user to fix parts of the image the were damaged during the montage process.

Photoshop
This section describes the photoshopping process for images. Before you start processing a large set of image it is recommended that you make a backup folder in case something goes wrong. It is considered best practice to leave the original image as an unaltered TIFF image in case you need to refer back to it. The original image should never be saved over.

Processing

 * Crop - Trims away unnecessary parts of the photo and allows the specimen to be better framed.
 * Levels - Adjusts the lighting levels to enhance the visibility of details. It improves contrast as well.
 * Sharpen/Smart sharpen - Detects and sharpens edges and removes blur.
 * Image Size - Pixel dimensions of the final images.
 * Export Format - The image format the final product is saved as.
 * Spot Healing Brush tool - Removes halo artifacts from around the specimens. A good montaging software can eliminate the need for this step.

How to make montage images through a microscope

Equipment A microscope with a trinocular head is preferred. If a trinocular head is not present, modifications will attach a camera to one of the eye pieces. This extra port allows the user to set up specimens using the eye pieces and then switch to the photo-tube for images. A microscope with a fine focus rail/stand in addition a coarse focus allows for more precise source images. A grey card (40% neutral) is a standard background in photography. Diffuse lighting can be achieved using several methods: 1) a light dome using LED lights can be attached to the microscope objective and pulled down over the specimen during images 2)

Example: Leica Application Suite

=Imaging Systems=

MCZ Leica Imaging System
System Components (used for ant images in Entomology Department, Museum of Comparative Zoology, Harvard University)


 * Microscope


 * Computer
 * PC Dell Studio XPS using Windows 7 Enterprise
 * Processor 2.93 GHz ; RAM 6 GB ;


 * Software
 * EOS Utility Canon Driver software
 * Helicon Focus 5.3 (frequent updates per year)
 * Photoshop CS6


 * Other
 * Diaphram set to maximum open position [Image]
 * Neutral Gray background card [Image]